rabbit polyclonal antibodies against runx3  (Cell Signaling Technology Inc)

Journal: Molecular Medicine Reports

Article Title: Loss of RUNX3 is significantly associated with advanced tumor grade and stage in endometrial cancers

doi: 10.3892/mmr.2018.8915

Figure Lengend Snippet: Expression of RUNX3 according to clinicopathological factors.

Article Snippet: To block endogenous peroxidase, the sections were incubated in 3% hydrogen peroxide in methanol at room temperature for 20 min. Next, sections were washed three times with distilled water, soaked in PBS pH 7.4 for 3 min, and then incubated with rabbit polyclonal antibody against human RUNX3 (39301; 1:100; Active Motif, Inc., Carlsbad, CA, USA) at room temperature for 1 h. Sections were washed with PBS three times for 3 min each and then were incubated with secondary antibody EnVision HRP-Labelled Polymer anti-rabbit (K4002; Dako; Agilent Technologies, Inc., Santa Clara, CA, USA) for 30 min at room temperature.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Loss of RUNX3 is significantly associated with advanced tumor grade and stage in endometrial cancers

doi: 10.3892/mmr.2018.8915

Figure Lengend Snippet: (A) Expression patterns of RUNX3 mRNA in two normal endometrial tissues, two endometrial cancer tissues, and two endometrial cancer cell lines, as determined by reverse-transcription-PCR. GAPDH was used as a standard reference (B) Methylation-specific PCR for the two endometrial cancer cell lines, HEC1-α and Ishikawa. RUNX3, runt-related transcription factor 3; PCR, polymerase chain reaction; N, normal endometrial tissue; T, endometrial cancer tissue; M, methylated; U, unmethylated; PC, positive control; NC, negative control.

Article Snippet: To block endogenous peroxidase, the sections were incubated in 3% hydrogen peroxide in methanol at room temperature for 20 min. Next, sections were washed three times with distilled water, soaked in PBS pH 7.4 for 3 min, and then incubated with rabbit polyclonal antibody against human RUNX3 (39301; 1:100; Active Motif, Inc., Carlsbad, CA, USA) at room temperature for 1 h. Sections were washed with PBS three times for 3 min each and then were incubated with secondary antibody EnVision HRP-Labelled Polymer anti-rabbit (K4002; Dako; Agilent Technologies, Inc., Santa Clara, CA, USA) for 30 min at room temperature.

Techniques: Expressing, Reverse Transcription, Methylation, Polymerase Chain Reaction, Positive Control, Negative Control

Journal: Molecular Medicine Reports

Article Title: Loss of RUNX3 is significantly associated with advanced tumor grade and stage in endometrial cancers

doi: 10.3892/mmr.2018.8915

Figure Lengend Snippet: Immunohistochemical staining of endometrial cancer tissues for RUNX3. (A) Grade 3 endometrial cancer sample displays negative staining (0). (B) Grade 2 endometrial cancer sample displays positive +1 staining (C) Grade 1 endometrial cancer displays +2 positive staining. (D) Grade 1 endometrial cancer displays +2 positive staining. Representative images at magnification, ×200. RUNX3, runt-related transcription factor 3.

Article Snippet: To block endogenous peroxidase, the sections were incubated in 3% hydrogen peroxide in methanol at room temperature for 20 min. Next, sections were washed three times with distilled water, soaked in PBS pH 7.4 for 3 min, and then incubated with rabbit polyclonal antibody against human RUNX3 (39301; 1:100; Active Motif, Inc., Carlsbad, CA, USA) at room temperature for 1 h. Sections were washed with PBS three times for 3 min each and then were incubated with secondary antibody EnVision HRP-Labelled Polymer anti-rabbit (K4002; Dako; Agilent Technologies, Inc., Santa Clara, CA, USA) for 30 min at room temperature.

Techniques: Immunohistochemical staining, Staining, Negative Staining

Journal: Molecular Medicine Reports

Article Title: Loss of RUNX3 is significantly associated with advanced tumor grade and stage in endometrial cancers

doi: 10.3892/mmr.2018.8915

Figure Lengend Snippet: Methylation-specific polymerase chain reaction analysis of the RUNX3 promoter was performed in six endometrial cancer tissues (T1-T6) and eight normal endometrial tissues (N1-N8). Positive findings were observed in T1-T4 and N4 samples. RUNX3, runt-related transcription factor 3; SM, size marker; C, control; M, methylated; U, unmethylated.

Article Snippet: To block endogenous peroxidase, the sections were incubated in 3% hydrogen peroxide in methanol at room temperature for 20 min. Next, sections were washed three times with distilled water, soaked in PBS pH 7.4 for 3 min, and then incubated with rabbit polyclonal antibody against human RUNX3 (39301; 1:100; Active Motif, Inc., Carlsbad, CA, USA) at room temperature for 1 h. Sections were washed with PBS three times for 3 min each and then were incubated with secondary antibody EnVision HRP-Labelled Polymer anti-rabbit (K4002; Dako; Agilent Technologies, Inc., Santa Clara, CA, USA) for 30 min at room temperature.

Techniques: Methylation, Polymerase Chain Reaction, Marker, Control

Journal: Molecular Medicine Reports

Article Title: Loss of RUNX3 is significantly associated with advanced tumor grade and stage in endometrial cancers

doi: 10.3892/mmr.2018.8915

Figure Lengend Snippet: Expression and methylation of RUNX3 according to clinicopathological factors.

Article Snippet: To block endogenous peroxidase, the sections were incubated in 3% hydrogen peroxide in methanol at room temperature for 20 min. Next, sections were washed three times with distilled water, soaked in PBS pH 7.4 for 3 min, and then incubated with rabbit polyclonal antibody against human RUNX3 (39301; 1:100; Active Motif, Inc., Carlsbad, CA, USA) at room temperature for 1 h. Sections were washed with PBS three times for 3 min each and then were incubated with secondary antibody EnVision HRP-Labelled Polymer anti-rabbit (K4002; Dako; Agilent Technologies, Inc., Santa Clara, CA, USA) for 30 min at room temperature.

Techniques: Expressing, Methylation

Journal: Molecular Medicine Reports

Article Title: Loss of RUNX3 is significantly associated with advanced tumor grade and stage in endometrial cancers

doi: 10.3892/mmr.2018.8915

Figure Lengend Snippet: Restoration of RUNX3 mRNA expression in HEC1-α cells following ADC treatment. Lane 1, 0 µM ADC control; lane 2, 0.5 µM ADC; lane 3, 1 µM ADC; lane 4, 5 µM ADC; lane 5, no template control. RUNX3, runt-related transcription factor 3; ADC, 5-aza-2′-deoxycytidine.

Article Snippet: To block endogenous peroxidase, the sections were incubated in 3% hydrogen peroxide in methanol at room temperature for 20 min. Next, sections were washed three times with distilled water, soaked in PBS pH 7.4 for 3 min, and then incubated with rabbit polyclonal antibody against human RUNX3 (39301; 1:100; Active Motif, Inc., Carlsbad, CA, USA) at room temperature for 1 h. Sections were washed with PBS three times for 3 min each and then were incubated with secondary antibody EnVision HRP-Labelled Polymer anti-rabbit (K4002; Dako; Agilent Technologies, Inc., Santa Clara, CA, USA) for 30 min at room temperature.

Techniques: Expressing, Control

Journal: PLoS ONE

Article Title: RUNX3 Mediates Suppression of Tumor Growth and Metastasis of Human CCRCC by Regulating Cyclin Related Proteins and TIMP-1

doi: 10.1371/journal.pone.0032961

Figure Lengend Snippet: a and b. Representative immunohistochemical photographs were taken at different magnifications in CCRCC tumor tissues and their matched noncancerous counterparts (×100 a1, ×200 a2, ×400 a3 and negative control a4 for noncancerous tissues; ×100 b1, ×200 b2, ×400 b3 and negative control b4 for tumor tissues). c. Expression protein levels of Runx3 in six CCRCC and the matched adjacent noncancerous tissues. d.Expression mRNA levels of RUNX3 in the CCRCC-derived cell lines and human kidney proximal tubular cell lines by real time RT-PCR. 18S was used as an internal control. * P <0.05 vs HKC cells. e. Expression protein levels of RUNX3 in the CCRCC-derived cell lines and human kidney proximal tubular cell lines by Western Blot. Tubulin was used as an internal control.

Article Snippet: Immunohistochemistry was done as described using a rabbit polyclonal antibody against human RUNX3 at a dilution of 1∶200 (Active Motif, Carlsbad, CA) .

Techniques: Immunohistochemical staining, Negative Control, Expressing, Derivative Assay, Quantitative RT-PCR, Control, Western Blot

Journal: PLoS ONE

Article Title: RUNX3 Mediates Suppression of Tumor Growth and Metastasis of Human CCRCC by Regulating Cyclin Related Proteins and TIMP-1

doi: 10.1371/journal.pone.0032961

Figure Lengend Snippet: Clinicopathological associations of RUNX3 expression in patients with CCRCC.

Article Snippet: Immunohistochemistry was done as described using a rabbit polyclonal antibody against human RUNX3 at a dilution of 1∶200 (Active Motif, Carlsbad, CA) .

Techniques: Expressing

Journal: PLoS ONE

Article Title: RUNX3 Mediates Suppression of Tumor Growth and Metastasis of Human CCRCC by Regulating Cyclin Related Proteins and TIMP-1

doi: 10.1371/journal.pone.0032961

Figure Lengend Snippet: a. After infection, the expression of RUNX3 in 786-O-Ctrl and 786-O-RUNX3 was evaluated by Western blot. Tubulin was used as an internal control. b. Effect of RUNX3 in regulating 786-O cells proliferation. Monolayer growth rates of 786-O, 786-O-Ctrl and 786-O-RUNX3 cells were determined by MTT assays. Values represent the mean (SEM) from at least three separate experiments. * P <0.05 vs 786-O cells. c. Effect of RUNX3 on colony formation of 786-O cells. Cells were placed in media containing soft agar and incubated for 17 days. The number of foci >100 µm was counted. Values represent the mean (SEM) from at least three separate experiments, each conducted in triplicate. * P <0.05 vs 786-O-Ctrl cells. d. Cell migration assays. Representative fields of migration cells on the membrane (magnification of ×200). Average migration cell number per field. The migration cell number of 786-O-RUNX3 is drastically decreased than that transfected with negative control. * P <0.05 vs 786-O-Ctrl cells, Student’s T-test, n = 10.

Article Snippet: Immunohistochemistry was done as described using a rabbit polyclonal antibody against human RUNX3 at a dilution of 1∶200 (Active Motif, Carlsbad, CA) .

Techniques: Infection, Expressing, Western Blot, Control, Incubation, Migration, Membrane, Transfection, Negative Control

Journal: PLoS ONE

Article Title: RUNX3 Mediates Suppression of Tumor Growth and Metastasis of Human CCRCC by Regulating Cyclin Related Proteins and TIMP-1

doi: 10.1371/journal.pone.0032961

Figure Lengend Snippet: a. Average tumor weight was measurement of the excised tumors at the time of sacrifice. * P <0.05 vs 786-O-Ctrl cells. b. Average tumor size was estimated by physical measurement of the excised tumor at different time. * P <0.05 vs 786-O-Ctrl cells. d. 786-O-Ctrl cells and 786-O-RUNX3 cells were cultured in DMEM for 24 h. Cells were harvested and processed for FACS analysis.

Article Snippet: Immunohistochemistry was done as described using a rabbit polyclonal antibody against human RUNX3 at a dilution of 1∶200 (Active Motif, Carlsbad, CA) .

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: RUNX3 Mediates Suppression of Tumor Growth and Metastasis of Human CCRCC by Regulating Cyclin Related Proteins and TIMP-1

doi: 10.1371/journal.pone.0032961

Figure Lengend Snippet: a. The expression of cyclin D1, cyclin E, cdk2, cdk4, p-Rb, Rb, p27, MMP2, MMP9, TIMP-1 and TIMP-2 proteins were evaluated in 786-O-Ctrl and 786-O-RUNX3 cells by Western blot. b. The expression of cyclin D1, cyclin E, cdk2, cdk4, p-Rb, Rb, p27, MMP2, MMP9, TIMP-1 and TIMP-2 proteins were evaluated in HKC-Ctrl and HKC-siRUNX3 by Western blot. All examined gene expression levels quantitatively analyzed and expressed as the ratios over β-actin.

Article Snippet: Immunohistochemistry was done as described using a rabbit polyclonal antibody against human RUNX3 at a dilution of 1∶200 (Active Motif, Carlsbad, CA) .

Techniques: Expressing, Western Blot, Gene Expression